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主营:抗体,重组蛋白,细胞培养基和分子生物学试剂盒
℡ 4000-520-616
℡ 4000-520-616
Usbio/G8965-09-ML550 Chicken Anti-Green Fluorescent Protein (GFP) (MaxLight 550)/G8965-09-ML550/100ul
产品编号:G8965-09-ML550
市  场 价:¥15780.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$789.00
品      牌: Usbio
公      司:United States Biological
公司分类:
Usbio/G8965-09-ML550 Chicken Anti-Green Fluorescent Protein (GFP) (MaxLight 550)/G8965-09-ML550/100ul
商品介绍

MaxLight™550isanewYellow-GreenphotostabledyeconjugatecomparabletoAlexaFluor™546,555,DyLight™549,Cy3™,TRITCandoffersbetterlabelingefficiency,brighterimagingandincreasedimmunodetection.Absorbance(550nm);Emission(575nm);ExtinctionCoefficient150,000.

Detectsabandofthepropermolecularweight,30kD,inlysatesfromE.coliexpressingGFP,butnotinE.colithatdonotexpressorinlysatespreparedfromE.colicontainingtheGFP-expressingplasmidbutnotinduced,detectGFPinlysatesfromCOSorprimaryneuronsorfibroblastcellstransfectedwithplasmidsdirectingexpressionGFPoratau-GFPfusionprotein.Didnotdetectanysignalwhen50ugofproteinfromaratbrainlysate(negativecontrol)wasfractionatedontheSDS-PAGE.
Applications:|SuitableforuseinWesternBlot,ImmunocytochemistryandImmunoprecipitation.Otherapplicationshavenotbeentested.
RecommendedDilutions
Immunocytochemistry:Cellswerefixedusing4%formaldehydeinPBS,pH7.4for20minutesatRT.ThecellsarepermeABIlizedwith0.2%TritonX-100and2%serum(correspondingtotheanimalinwhichthesecondaryantibodywasraised)inPBSfor20minutesatRT.CellculturesarethenincubatedwithprimaryantiserainPBScontaining2%serum(asabove),at4°C,overnight.Hasalsobeenusedsuccessfullywiththeavidin/biotin/peroxidasedetectionsystem.Cultureswerewashedfourtimes(10minuteseach)betweensteps,andreactionproductisdevelopedwitha0.05%diaminobenzidine/0.02%hydrogenperoxidesolutiononicefor8-10minutes.Optimaldilutionstobedeterminedbytheresearcher.
StorageandStability
Storeproductat4°Cinthedark.DONOTFREEZE!Stableat4°Cfor12monthsafterreceiptasanundilutedliquid.Diluterequiredamountonlypriortoimmediateuse.Furtherdilutionscanbemadeinassaybuffer.Caution:MaxLight™550conjugatesaresensitivetolight.Formaximumrecoveryofproduct,centrifugetheoriginalvialpriortoremovingthecap.
Note:Applicationsarebasedonunconjugatedantibody.
References
1.Hallam,S.J.,etal.,NatureNeuroscience5:1137-1146(2002).2.Sippy,T.,etal.,NatureNeuroscience10:1031-1038(2003).3.Jakobsson,J.,etal.,EuropeanJournalofNeuroscience19:761-765(2004).4.Zhao,S.,etal.,EuropeanJournalofNeuroscience19:1133-1140(2004).5.Yawata,S.,etal.,JournalofNeuroscience26:3626-3633(2006).
品牌介绍
USbio全称United States Biological,位于麻省斯万普斯科特(Swampscott, MA)。全美**的抗原抗体和生化试剂供应商,目前向全球六十余个国家的科研工作者提供约50,000种抗体、抗原和生化试剂,以质量**闻名。主要产品种类包括:抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国US Biological公司是生物化学和生物学试剂的专业生产商,产品服务于免疫学,分子生物学,分子遗传学,体外诊断,组织培养等科研领域。产品精确度高,质量稳定,产品数量巨大,提供70,000多种抗体、抗原和生化试剂等。
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