CathepsinBisacysteineproteasecommonlyoverexpressedinmetastisizingcancercells.Anintracellular(lysosomal)enzymeinpeptidasefamilyC1(papainfamily),CathepsinBwillhydrolyzeproteinswithbroadspecificityforpeptidebonds.Preferentiallycleaves-Arg-Argbondsinsmallmoleculesubstrates(thusdifferingfromcathepsinL).Inadditiontobeinganendopeptidase,showspeptidyl-dipeptidaseactivity,liberatingC-terminaldipeptides.
Applications:|Suitableforusein
ELISAandWesternBlot.Otherapplicationsnottested.
RecommendedDilutions
ELISA(Indirect):1:1024WesternBlot:1:4000Optimaldilutionstobedeterminedbytheresearcher.
Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor12monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
Note:Applicationsarebasedonunconjugatedantibody.
Immunogen
CathepsinB(Humanliver).
Form
SuppliedasaliquidinPBS,pH7.2.Nopreservativeadded.LabeledwithR-Phycoerythrin(PE).
Specificity
RecognizeshumanliverCathepsinB.DoesnotcrossreactwithCathepsinL,CathespinDorCathepsinH.