MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (759nm); Emission (780nm); Extinction Coefficient 240,000.
Sirtuin (SIRT5) is known as a nicotinamide adenine dinucleotide- dependent deacetylase that is a member of the silent information regulator 2 (Sir2) family of proteins that is important in aging, metabolism and disease. SIRT5 is expressed in a variety of tissues. Two different isoform have been described. Isoform 1 is reported to be express in the mitochondria and cytoplasm while isoform 2 appears to be localized to the mitochondria only. SIRT5 has also been linked to many primate-specific functions. SIRT5 has recently been implicated as having a role in adaptation to fasting, caloric restriction and high protein diets.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5ug/mlImmunohistochemistry: 1:500Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic linear peptide corresponding to human SIRT5 (KLH). Species sequence homology: chimpanzee and rhesus macaque (100%).
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SIRT5. Species Crossreactivity: rat.
References
1. Matsushita, N., et al. (2011). Genes Cells. 16(2):190-202. 2. Nakagawa, T. and Guarente, L. (2009). Aging (Albany NY). 1(6):578-581. 3. Mahlknecht, U., et al. (2006). Cytogenet Genome Res. 112(3-4):208-212.