5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming. |5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammilian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation.

Suitable for use in Dot Blot, Immunoprecipitation.

Dot Blot: 1:5000 detected only 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA.

Immunoprecipitation: Immunoprecipitates 5-Hydroxymethylcytosine DNA using chomatin from 293T cells overexpressing mTET1-CD. (Dr. D. Chen, Shanghai Institute of Biological Sciences)

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to stain 5-Hydroxymethylcytosine DNA in cortical neurons from atg5-disrupted mouse. (Dr. D. Chen, Shanghai Institute of Biological Sciences)

Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

BSA-conjugated recombinant Hydroxymethylcytosine DNA.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.

Purified by Protein G affinity chromatography.

Detects 5-hydroxymethyl cytosine, but not 5-methylcytosine or unmethylated cytosine. Non-species specific antibody against modified DNA. Expected to detect 5-hydroxymethylcytosine in all species.