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主营:抗体,重组蛋白,细胞培养基和分子生物学试剂盒
℡ 4000-520-616
℡ 4000-520-616
Usbio/H1847-52X-AP Rabbit Anti-Hemagglutinin Tag (CYPYDVPDYASL) (HA Tag) (BSA & Azide Free) (AP)/H1847-52X-AP/100ul
产品编号:H1847-52X-AP
市  场 价:¥16980.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$849.00
品      牌: Usbio
公      司:United States Biological
公司分类:
Usbio/H1847-52X-AP Rabbit Anti-Hemagglutinin Tag (CYPYDVPDYASL) (HA Tag) (BSA & Azide Free) (AP)/H1847-52X-AP/100ul
商品介绍

Asmallsegmentoftheviralhemagglutinincoatproteiniswidelyusedasanepitopetagtofacilitatepurificationandfunctionalanalysisofproteinsofinterest.EpitopetagsareshortpeptidesequencesthatareeasilyrecognizedbyreADIlyavailabletag-specificantibodies.Ligationofepitope-tagencodingDNAtoclonedDNAsofinterestproducesepitopetaggedfusionproteinsthatcanthenbesubjectedtoantibody-dependentexperimentalprocedures.Epitopetaggingtechniquesarewidelyusedinthepurification,identification,andfunctionalanalysisofproteins.Expressionvectorsforproducingtagfusionproteinsinavarietyoforganisms(includingbacteria,yeast,insect,andmammaliancells)arecommerciallyavailable.Someofthemorecommonlyusedfusiontagsinclude:c-myc,GFP,GST,HA,His,amdMBP.

Applications
SuitableforuseinWesternBlotandImmunoprecipitation.Otherapplicationsnottested
RecommendedDilutions
WesternBlot:5-10ug/mlfor2hoursatRT.Immunoprecipitation:10ug/mgproteinlysate.UseProteinA.Optimaldilutionstobedeterminedbytheresearcher.
PositiveControl:|ProteinsdisplayingtheYPYDVPDYAHA-tag
StorageandStABIlity
Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor12months.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap. 
Note:Applicationsarebasedonunconjugatedantibody.
References
1.Terpe,K.,Appl.Microbiol.Biotechnol.60:523-533(2003).2.Tu,Y.,etal.,Mol.Biol.Cell9:3367-3382(1998).3.BhattacharyaandCabral,Mol.Biol.Cell15(7):3123-3131(2004).4.Yamanaka,etal.,Curr.Biol.13(9):734-743(2003).5.Chang,etal.,GenesDev.18(16):2010-2023(2004).6.Munro,S.,Pelham,H.R.,EMBOJ.3:3087-3093(1984).7.Madeo,F.,etal.,Mol.Cell9:911-917(2002).8.Ruckdeschel,K.,etal.,J.Immunol.168:4601-4611(2002).9.Field,J.,etal.,Mol.Cell.Biol.8:2159-2165(1988).10.Schirmer,E.,etal.,J.CellBiol.153:479-490(2001).11.Bjerling,P.,etal.,Mol.Cell.Biol.8:2170-2181(2002).12.Wilson,l.A.,etal.,Cell37:767-78(1984).13.Muster,T.,etal.,J.Virol.69:6678-6686(1995).
品牌介绍
USbio全称United States Biological,位于麻省斯万普斯科特(Swampscott, MA)。全美**的抗原抗体和生化试剂供应商,目前向全球六十余个国家的科研工作者提供约50,000种抗体、抗原和生化试剂,以质量**闻名。主要产品种类包括:抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国US Biological公司是生物化学和生物学试剂的专业生产商,产品服务于免疫学,分子生物学,分子遗传学,体外诊断,组织培养等科研领域。产品精确度高,质量稳定,产品数量巨大,提供70,000多种抗体、抗原和生化试剂等。
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