Asmallsegmentoftheviralhemagglutinincoatproteiniswidelyusedasanepitopetagtofacilitatepurificationandfunctionalanalysisofproteinsofinterest.EpitopetagsareshortpeptidesequencesthatareeasilyrecognizedbyreADIlyavailabletag-specificantibodies.Ligationofepitope-tagencodingDNAtoclonedDNAsofinterestproducesepitopetaggedfusionproteinsthatcanthenbesubjectedtoantibody-dependentexperimentalprocedures.Epitopetaggingtechniquesarewidelyusedinthepurification,identification,andfunctionalanalysisofproteins.Expressionvectorsforproducingtagfusionproteinsinavarietyoforganisms(includingbacteria,yeast,insect,andmammaliancells)arecommerciallyavailable.Someofthemorecommonlyusedfusiontagsinclude:c-myc,GFP,GST,HA,His,amdMBP.
Applications
SuitableforuseinWesternBlotandImmunoprecipitation.Otherapplicationsnottested
RecommendedDilutions
WesternBlot:5-10ug/mlfor2hoursatRT.Immunoprecipitation:10ug/mgproteinlysate.UseProteinA.Optimaldilutionstobedeterminedbytheresearcher.
PositiveControl:|ProteinsdisplayingtheYPYDVPDYAHA-tag
Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor12months.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
Note:Applicationsarebasedonunconjugatedantibody.
References
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