JAM2, also known as CD322, belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. JAM2 is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs.
Applications: |Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
F0 myeloma cells with spleen cells from BALB/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa21-238 from human JAM2, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human JAM2.
References
1. Zhang Z., et al. (2004) Protein Sci. 13:2819-2824.|2. Liu T., et al. (2005) J. Proteome Res. 4:2070-2080.