GlycoproteinB(gB)isthemainantigenicproteinwithintheenvelopeofHCMV.ItisrequiredforCMVmediatedmembranefusionalongwiththegH/gLcomplex.TheunprocessedprecursorofHCMVgBis906aainlength,withaMWof~100kD.
Applications
Suitableforusein
ELISAandImmunofluorescence.Otherapplicationsnottested.
RecommendedDilutions
Immunofluorescence:Useacetone-fixedCMVinfectedcellsorfrozensections.Optimaldilutionstobedeterminedbytheresearcher.
RecommendedSecondaryAntibodies
I1904-06C:IgG,H&L(HRP)(X-Adsorbed)PabGtxMoI1904-06H:IgG,H&L(HRP)(X-Adsorbed)PabGtxMo
Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor12monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
Note:Applicationsarebasedonunconjugatedantibody.
Immunogen
HumanCMVextracts.
Form
SuppliedasaliquidinPBS,pH7.2.LabeledwithFluoresceinisothiocyanate(FITC).
Purity
PurifiedbyProteinAaffinitychromatography.
Specificity
RecognizesCytomegalovirusGlycoproteinB,LateNeutralizingAntigen.ReactswithMRC-5infectedcellsbutnotuninfectedcells.Thisantibodyhasbeenfoundtobeneutralizing.