Sample Type:|Serum, plasma, tissue homogenates and other biological fluids||Intended Use:|The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNFRSF8 in rat serum, plasma, tissue homogenates and other biological fluids.||Sensitivity:|0.135ng/ml||Range:|0.312-20ng/ml||Specificity:|This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8).|No significant cross-reactivity or interference between Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) and analogues was observed.||Precision:|Intra-Assay: CV<10% |Inter-Assay: CV<12%||Test Principle:|The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNFRSF8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TNFRSF8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFRSF8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNFRSF8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.||Kit Components:|96-well strip plate, 1x Plate |Standard, 2x vial|Detection Reagent A, 1×120ul|Detection Reagent B, 1×120ul|TMB Substrate, 1×9ml|Wash Buffer (30X), 1×20ml|Standard Diluent, 1×20ml|Assay Diluent A, 1×12ml|Assay Diluent B, 1×12ml|Stop Solution, 1x6ml||Storage and Stability:|Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

USbio全称United States Biological，位于麻省斯万普斯科特(Swampscott, MA)。全美**的抗原抗体和生化试剂供应商，目前向全球六十余个国家的科研工作者提供约50,000种抗体、抗原和生化试剂，以质量**闻名。主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国US Biological公司是生物化学和生物学试剂的专业生产商，产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。产品精确度高，质量稳定，产品数量巨大，提供70,000多种抗体、抗原和生化试剂等。