TransfectionReagent(invivo)ishighlyeffectiveforDNAplasmiddeliveryinvivo,whilenotinducinganinflammatoryresponse.TurboFectinvivohasbeenassessedforplasmidDNAdeliveryinbothmiceandratsandissuitableforDNAadmiNISTrationviavariousroutes,includingintravenous,intraperitoneal,andintratumoralinjection.TheamountofDNAandsuggestedinjectionvolumewilldependontheexperimentalanimalandtherouteofadministrationaswellasthetargetedtissueororganandtheexpressionvector.||TransfectionReagent(invivo)isasterilesolutionofacationicpolymerinwater.Thepolymerformscompact,stable,positivelychargedcomplexeswithDNA.ThesecomplexesprotectDNAfromdegradationandfacilitategenedeliveryinvivo.ReagentcausesnodetectableinflamatoryresponseandissuitableforDNAadministrationviavariousroutes,includingintravenous,intraperitoneal,intratumoralinjection,etc.||TransfectionReagent(invivo)for20transfectionsusing50ugDNA.||ReagentsRequiredbutnotSupplied:|Sterile5%glucosesolutionfordilutionofDNA.||GeneralConsiderations:||DNAQualityRequirements:|DNAqualityiscriticalforsuccessfultransfection.AnA260/A280ratioof1.8orhigherisrecommended.DNAshouldbesterileandfreeofanycontaminantssuchasendotoxins.||ChoiceofPromoter:|Highgeneexpressiondependsonboththepromoterunderwhichthegeneofinterestisexpressedandthetargetedtissue/organ.Cytomegalovirus(CMV)promoterisbestknownforhighgeneexpressioninawidevarietyofcells.SomeresearcherspreferLTR,simianvirus(SV40)orRoussarcomavirus(RSV)promoters.||TransfectionReagent/DNARatio:|ThevolumeoftransfectionreagentuseddependsontheamountofDNA,transgeneandanimalstobetransfected.Theratiospresentedintheprotocolsbelowarestartingamountsandcanbefurtheroptimizedforbestresults.||AmountofDNAandsuggestedinjectionvolume:|TheamountofDNAandsuggestedinjectionvolumedependontheexperimentalanimalandtherouteofadministration(seeprotocol)aswellasthetargetedtissueororganandtheexpressionvector.TopreventprecipitationofTransfectionReagent/DNAcomplexes,thefinalconcentrationofDNAintheinjectionmixshouldnotexceed0.5ug/ul.||QualityControl:|TransfectionefficiencywastestedonBALB/cfemalemice(7-8weeksold)using50ugFireflyluciferasecodingplasmidand6ulofinvivoTransfectionreagentspermouse.ThedeliveryofthetransgenewasevaluatedbymeasurementofFireflyluciferaseactivityintotalproteinextractedfromthelungsoftransfectedanimals.100%oftestedmiceexpressedtheFireflyluciferase.||StorageandStABIlity:|Maybestoredat4°C.Forlong-termstorage,aliquotandstoreat4°C.Donotfreeze.Aliquotsarestablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialpriortoremovingthecap.

USbio全称United States Biological，位于麻省斯万普斯科特(Swampscott, MA)。全美**的抗原抗体和生化试剂供应商，目前向全球六十余个国家的科研工作者提供约50,000种抗体、抗原和生化试剂，以质量**闻名。主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国US Biological公司是生物化学和生物学试剂的专业生产商，产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。产品精确度高，质量稳定，产品数量巨大，提供70,000多种抗体、抗原和生化试剂等。