MaxLight™650isanewFar-IRstabledyeconjugatecomparabletoAlexaFluor™647,DyLight™649,Cy5™andoffersbetterlabelingefficiency,brighterimagingandincreasedimmunodetection.Absorbance(655nm);Emission(676nm);ExtinctionCoefficient250,000.
Y.pestishasanumberofvirulencefactorsthatenableittosurviveinhumansbyfacilitatinguseofhostnutrients,causingdamagetohostcells,andsubvertingphagocytosisandotherhostdefensemechanisms.CapsularF1antigenisamainimmunochemicalcomponentofY.pestissurface.ItssynthesisisdeterminedbypFraplasmidandinducedat370C.F1isaggregatedontheoutermembraneoftheY.pestismicrobeasanolygomericprotein,formingagranularlayerandgraduallydiffusingintotheenvironment.Capsularpolymerconsistsofmultiplesimilarhydrophobicproteinsubunits,aggregatingatphysiologicalconditions.F1subunitoftheantigenisoriginallysynthesizedfrom170aminoacidsandhasamolecularmass17.6Kd,then,aftertheeliminationofthesignalpeptideduringthesecretionontothesurface,aproteinwithmolecularmass15.6Kdandisoelectricpoint4.1isformed.
Applications:|SuitableforuseinImmunohistochemistryandFLISA.Otherapplicationsnottested.
RecommendedDilutions
Optimaldilutionstobedeterminedbytheresearcher.
Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor12months.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
Note:Applicationsarebasedonunconjugatedantibody.