Y. pestis has a number of virulence factors that enable it to survive in humans by facilitating use of host nutrients, causing damage to host cells, and subverting phagocytosis and other host defense mechanisms. Capsular F1 antigen is a main immunochemical component of Y. pestis surface. Its synthesis is determined by pFra plasmid and induced at 370C. F1 is aggregated on the outer membrane of the Y. pestis microbe as an olygomeric protein, forming a granular layer and gradually diffusing into the environment. Capsular polymer consists of multiple similar hydrophobic protein subunits, aggregating at physiological conditions. F1 subunit of the antigen is originally synthesized from 170 amino acids and has a molecular mass 17.6kD, then, after the elimination of the signal peptide during the secretion onto the surface, a protein with molecular mass 15.6kD and isoelectric point 4.1 is formed.
Applications: |Suitable for use in Immunofluorescence, Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Hybridoma: |Sp2/0 myeloma cells with spleen cells from BALB/c mice
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified F1 antigen of Y. pestis vaccine strain EB.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes the capsule bearing strains of Y. pestis. Does not react with Y. pseudotuberculosis and Y. enterocolitica.