Thrombospondin-1 is believed to play a role in cell migration and proliferation, during embryogenesis and wound repair. It has also been used as a potential regulator of tumor growth and metastasis. TSP expression is highly regulated by different hormones and cytokines and is developmentally controlled. TSP stimulates the growth of vascular smooth muscle cells and human foreskin fibroblasts. A combination of IFN-gamma and TNF-alpha inhibits TSP production in these cells.
Applications: |Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:200Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human TSP1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% BSA, 0.01% thimerosal, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human TSP1.