InitialstudiessuggestedthatDAImaybeinvolvedinprocessessuchhostresponsetoneoplasia,additionalresearchhasindicatedthatthecytosolicDAIcanactlikethetoll-likereceptorTLR9bydetectingcytosolicdouble-stranded(ds)DNAandtriggerinductionoftypeIinterferonandotherinnateimmuneresponses.ItisthoughtthatthebindingofDAItodsDNAenhancesitsassociationwithinnateimmuneresponseproteinssuchastheIRF3transcriptionfactorandtheserine/threoninekinaseTBK1(alsoknownasNAK).
DAIishighlyexpressedinlymphatictissuessuchasthelymphnode,leukocytes,tonsil,bonemarrowandspleen.Expressionatalowerlevelisalsoseeminthymus,lungandliver.IthasbeendemonstratedthatmultipleisoformsofDAIexist.
Applications
Suitableforusein
ELISAandWesternBlot.Otherapplicationsnottested.
RecommendedDilution
WesternBlot:0.5-1ug/mlOptimaldilutionstobedeterminedbytheresearcher.
Maybestoredat4°Cforshort-termonly.Forlong-termstorageandtoavoidrepeatedfreezingandthawing,addsterile40-50%glycerol,aliquotandstoreat-20°C.Aliquotsarestableforatleast12monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.
Note:Applicationsarebasedonunconjugatedantibody.
Immunogen
Syntheticpeptidecorrespondingtoan18aasequencefromnearthecarboxyterminusofhuman.
Form
SuppliedasaliquidinPBS,pH7.2.LabeledwithBiotin.
Purity
Purifiedbyimmunoaffinitychromatography.
Specificity
RecognizeshumanDAI.SpeciesCrossreactivity:mouseandrat.
References
1.TakaodaAetal(2007)DAI(DLM-1/ZBP1)isacytosolicDNAsensorandanactivatorofinnateimmuneresponse.Nature448:501-5.2.SchwartzT,etal.(2001)StructureoftheDLM-1-Z-DNAcomplexrevealsaconservedfamilyofZ-DNA-bindingproteins.Nat.Struct.Biol.8:761-5.