MaxLight™ 750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (759nm); Emission (780nm); Extinction Coefficient 240,000.
ZWINT is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells.
Applications: |Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
FLISA: 1:1,000Flow Cytometry: 1:10-1:50Immunohistochemistry: 1:50-1:100Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher.Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide between amino acids 66-96 in the center region of human ZWINT
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human ZWINT. Species Crossreactivity: mouse.
References
1. Famulski,J.K., J. Cell Biol. 180(3), 507-520 (2008). 2. Kops,G.J., J. Cell Biol. 169(1), 49-60 (2005). 3. Wang,H., J. Biol. Chem. 279(52), 54590-54598 (2004).