RNA-directedRNApolymerasethatcatalyzesthetranscriptionofviralmRNAs,theircappingandpolyadenylation.ThetemplateiscomposedoftheviralRNAtightlyencapsidatedbythenucleoprotein(N).TheviralpolymerasebindstothegenomicRNAatthe3'leaderpromoter,andtranscribessubsequentlyallviralmRNAswithadecreasingefficiency.Thefirstgeneisthemosttranscribed,andthelasttheleasttranscribed.Theviralphosphoproteinactsasaprocessivityfactor.CappingisconcommitantwithinitiationofmRNAtranscription.Indeed,aGDPpolyribonucleotidyltransferase(PRNTase)addsthecapstructurewhenthenascentRNAchainlengthhasreachedfewnucleotides.Ribose2'-OmethylationofviralmRNAcapprecedesandfacilitatessubsequentguanine-N-7methylation,bothactivitiesbeingcarriedbytheviralpolymerase.PolyadenylationofmRNAsoccurbyastutteringmechanismatasliperystopsitepresentattheendviralgenes.AfterfinishingtranscriptionofamRNA,thepolymerasecanresumetranscriptionofthedownstreamgene.RNA-directedRNApolymerasethatcatalyzesthereplicationofviralgenomicRNA.ThetemplateiscomposedoftheviralRNAtightlyencapsidatedbythenucleoprotein(N).ThereplicasemodeisdependentonintracellularNproteinconcentration.Inthismode,thepolymerasereplicatesthewholeviralgenomewithoutrecognizingtranscriptionalsignals,andthereplicatedgenomeisnotcapedorpoly.||Source:|Partialrecombinantproteincorrespondingtoaa625-809ofZaireEbolavirus(strainMayinga-76)RNA-DirectedRNAPolymeraseL,fusedto6xHis-TagatN-terminal,expressedinE.coli.||MolecularWeight:|~24.9kD||AASequence:|RGSSFVTDLEKYNLAFRYEFTAPFIEYCNRCYGVKNVFNWMHYTIPQCYMHVSDYYNPPHNLTLENRDNPPEGPSSYRGHMGGIEGLQQKLWTSISCAQISLVEIKTGFKLRSAVMGDNQCITVLSVFPLETDADEQEQSAEDNAARVAASLAKVTSACGIFLKPDETFVHSGFIYFGKKQYLNG||StorageandStABIlity:|Maybestoredat4°Cforshort-termonly.Aliquottoavoidrepeatedfreezingandthawing.Storeat-20°C.Aliquotsarestablefor6monthsafterreceiptat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.

USbio全称United States Biological，位于麻省斯万普斯科特(Swampscott, MA)。全美**的抗原抗体和生化试剂供应商，目前向全球六十余个国家的科研工作者提供约50,000种抗体、抗原和生化试剂，以质量**闻名。主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国US Biological公司是生物化学和生物学试剂的专业生产商，产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。产品精确度高，质量稳定，产品数量巨大，提供70,000多种抗体、抗原和生化试剂等。