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主营:抗体,重组蛋白,细胞培养基和分子生物学试剂盒
℡ 4000-520-616
℡ 4000-520-616
Usbio/519272  Anti-Gpx2 (Glutathione Peroxidase 2) BioAssay™ ELISA Kit (Rat)/96Tests/519272
产品编号:519272
市  场 价:¥15580.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$779.00
品      牌: Usbio
公      司:United States Biological
公司分类:
Usbio/519272  Anti-Gpx2 (Glutathione Peroxidase 2) BioAssay™ ELISA Kit (Rat)/96Tests/519272
商品介绍
The Gpx2 (Glutathione Peroxidase 2) BioAssay™ ELISA Kit (Rat) is a quantitative sandwich assay for the detection of Gpx2 in serum, plasma, tissue homogenates and other biological fluids.||Sensitivity:|0.094ng/ml||Detection Range:|0.156-10ng/ml||Specificity:|This assay has high sensitivity and excellent specificity for detection of rat Gpx2. No significant cross-reactivity or interference between Gpx2 and analogues was observed.||Precision:|Intra-Assay: CV<8%|Inter-Assay: CV<10%||Test Principle:|This ELISA kit employs the sandwich enzyme-linked immune-sorbent assay technology. Anti-Gpx2 antibody was pre-coated onto 96-well plates. The biotin conjugated anti-Gpx2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Gpx2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Gpx2 can be calculated.||Kit Components:|Micro ELISA Plate(Dismountable), 8×12|Lyophilized Standard, 2x vial|Sample / Standard dilution buffer, 1x20ml|Biotin-labeled antibody (Concentrated), 1x120ul|Antibody dilution buffer, 1x10ml|HRP-Streptavidin Conjugate(SABC), 1x120ul|SABC dilution buffer, 1x10ml|TMB substrate, 1x10ml|Stop solution, 1x10ml|Wash buffer (25X), 1x30ml|Plate Sealer, 1x5pieces||Storage and Stability:|Store unopened kit at 4ºC; store at -20ºC once opened. Store unopened standard at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
品牌介绍
USbio全称United States Biological,位于麻省斯万普斯科特(Swampscott, MA)。全美**的抗原抗体和生化试剂供应商,目前向全球六十余个国家的科研工作者提供约50,000种抗体、抗原和生化试剂,以质量**闻名。主要产品种类包括:抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国US Biological公司是生物化学和生物学试剂的专业生产商,产品服务于免疫学,分子生物学,分子遗传学,体外诊断,组织培养等科研领域。产品精确度高,质量稳定,产品数量巨大,提供70,000多种抗体、抗原和生化试剂等。
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