C-RPQIRQKIENKPLQE,fromtheinternalregionofhumanZEB1.|Controlpeptidecorrespondingtopolyclonalantibody,Catalog#Z0469-75A,goatxhuman.||ZEB1inhibitsinterleukin-2(IL-2)geneexpression.MayberesponsIBLefortranscriptionalrepressionoftheIL-2gene.EnhancesorrepressesthepromoteractivityoftheATP1A1genedependingonthequantityofCDNAandonthecelltype.DefectsinZEB1arethecauseofposteriorpolymorphouscornealdystrophytype3(PPCD3)[MIM:609141].Posteriorpolymorphouscornealdystrophy(PPCD)isararediseaseinvolvingmetaplasiaandovergrowthofcornealendothelialcells.InpatientswithPPCD,thesecellsmanifestinanepithelialmorphologyandgeneexpressionpattern,produceanaberrantbasementmembrane,and,sometimes,spreadovertheirisandnearbystructuresinawaythatincreasestheriskforglaucoma.||Applications:|SuitableforuseinELISA.Otherapplicationsnottested.||RecommendedDiltuinos:|Optimaldilutionstobedeterminedbytheresearcher.||StorageandStABIlity:|Maybestoredat4°Cforshort-termonly.Forlong-termstorageandtoavoidrepeatedfreezingandthawing,aliquotandstoreat-20°C.Aliquotsarestableforatleast12monthsat-20°C.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.Furtherdilutionscanbemadeinassaybuffer.||PeptideBlocking(seecorrespondingantibodyZ0469-75A):|Antibodiesaretypicallysuppliedat0.5mg/mlandpeptidesasa100ulpellet.Whenpeptidesarereconstitutedin200ulwater,theconcentrationwouldbealso0.5mg/ml.Tostart,thebestratiowouldbe1:1(whichmeansmolarexcessofpeptidesrelativetoantibodieswhenidenticalvolumesaremixed).Mixequalvolumesofpeptideandantibodyattherequireddilutionandleaveatambienttemperature.Itisbestistohavetwoidenticalblots,tobeincubatedwithequalamountofantibodies,butonewiththeantibodiespre-adsorbedtothepeptidefor20min.Thenincubateanddevelopthetwoblotsinparallel.

USbio全称United States Biological，位于麻省斯万普斯科特(Swampscott, MA)。全美**的抗原抗体和生化试剂供应商，目前向全球六十余个国家的科研工作者提供约50,000种抗体、抗原和生化试剂，以质量**闻名。主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国US Biological公司是生物化学和生物学试剂的专业生产商，产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。产品精确度高，质量稳定，产品数量巨大，提供70,000多种抗体、抗原和生化试剂等。